Neuropeptides
Volume 46, Issue 1 , Pages 19-27 , February 2012

Changes in galanin and GalR1 gene expression in discrete brain regions after transient occlusion of the middle cerebral artery in female rats

  • Lovisa Holm

      Affiliations

    • Division of Clinical Chemistry, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden
    • ,
  • Susanne Hilke

      Affiliations

    • Division of Clinical Chemistry, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden
    • ,
  • Csaba Adori

      Affiliations

    • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    • ,
  • Elvar Theodorsson

      Affiliations

    • Division of Clinical Chemistry, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden
    • ,
  • Tomas Hökfelt

      Affiliations

    • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
    • ,
  • Annette Theodorsson

      Affiliations

    • Division of Clinical Chemistry, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden
    • Division of Neurosurgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden
    • Corresponding Author InformationCorresponding author at: Division of Neurosurgery, Department of Clinical and Experimental Medicine, Linköping University, SE-581 85 Linköping, Sweden. Tel.: +46 (0)101037585; fax: +46 (0)101033240.

Received 20 June 2011 ,Accepted 27 November 2011.

  • Image Result

    Lesion areas of coronal slices of the brains of female rats visualized by TTC staining after transient (60min) occlusion of the middle cerebral artery. The TTC staining colors viable (well-functioning

    Lesion areas of coronal slices of the brains of female rats visualized by TTC staining after transient (60min) occlusion of the middle cerebral artery. The TTC staining colors viable (well-functioning mitochondria) tissue in red and ischemic tissue lesions in white.

  • Image Result
    Areas of ischemic lesions in coronal slices of the brains of female rats determined with the TTC staining method 7days after transient (60min) occlusion of the middle cerebral artery. The lesions were

    Areas of ischemic lesions in coronal slices of the brains of female rats determined with the TTC staining method 7days after transient (60min) occlusion of the middle cerebral artery. The lesions were largest at B (6.2±1.7% and B −2mm (5.7±0.9%).

  • Image Result
    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from locus coeruleus in female rats (n=22) 7days after 60min transient occlusion of the midd

    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from locus coeruleus in female rats (n=22) 7days after 60min transient occlusion of the middle cerebral artery. The galanin gene expression in the locus coeruleus was up-regulated by 259% (p<0.01) and GalR1 by 45% (p<0.05) in the ischemic hemisphere as compared to the contra-lateral control side.

  • Image Result
    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from parietal cortex in female rats (n=22) 7days after 60min transient occlusion of the midd

    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from parietal cortex in female rats (n=22) 7days after 60min transient occlusion of the middle cerebral artery. In cortex, gene expression of GalR1 decreased by 35% (p<0.05) in the ischemic hemisphere as compared to the contra-lateral control side.

  • Image Result
    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from dorsal of hippocampus in female rats (n=22) 7days after 60min transient occlusion of th

    Gene expression of galanin, galanin receptor1 (GalR1), GalR2 and GalR3 measured by RT-PCR in punch biopsies from dorsal of hippocampus in female rats (n=22) 7days after 60min transient occlusion of the middle cerebral artery.

  • Image Result
    Immunofluorescence micrographs of three different rostro-caudal levels from locus coeruleus of a control female rat (A, C, and E) and a female rat 7days after a 60min transient occlusion of the middle

    Immunofluorescence micrographs of three different rostro-caudal levels from locus coeruleus of a control female rat (A, C, and E) and a female rat 7days after a 60min transient occlusion of the middle cerebral artery (B, D, F) after incubation with galanin antiserum. Note: strongly fluorescent cell bodies and processes in controls (A, C, and E) versus weak fluorescence in stroke rat (B, D, and F). Bar indicates 100μm.

PII: S0143-4179(11)00103-X

doi: 10.1016/j.npep.2011.11.001

Neuropeptides
Volume 46, Issue 1 , Pages 19-27 , February 2012

Article Tools

* Image Usage & Resolution