Neuropeptides
Volume 44, Issue 1 , Pages 17-23 , February 2010

Differential effect of prolonged food restriction and fasting on hypothalamic malonyl-CoA concentration and expression of orexigenic and anorexigenic neuropeptides genes in rats

  • Elzbieta Sucajtys-Szulc

      Affiliations

    • Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Jacek Turyn

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Elzbieta Goyke

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Justyna Korczynska

      Affiliations

    • Department of Pharmaceutical Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Ewa Stelmanska

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Ewa Slominska

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Ryszard T. Smolenski

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Boleslaw Rutkowski

      Affiliations

    • Department of Nephrology, Transplantology and Internal Medicine, Medical University of Gdansk, 80-211 Gdansk, Poland
    • ,
  • Julian Swierczynski

      Affiliations

    • Department of Biochemistry Medical University of Gdansk, 80-211 Gdansk, Poland
    • Corresponding Author InformationCorresponding author. Tel.: +48 58 349 1462; fax: +48 58 349 1465.

Received 3 June 2009 ,Accepted 12 November 2009.

References 

  1. Archer ZA, Moar KM, Logie TJ, Reilly L, Stevens V, Morgan PJ, et al. Hypothalamic neuropeptide gene expression during recovery from food restriction superimposed on short-day photoperiod-induced weight loss in the Siberian hamster. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. 2007;293:R1094–R1101
  2. Brady LS, Smith MA, Gold PW, Herkenham M. Altered expression of hypothalamic neuropeptide mRNAs in food-restricted and food-deprived rats. Neuroendocrinology. 1990;52:441–447
  3. Chakravarthy MV, Zhu Y, Lopez M, Yin L, Wozniak DF, Coleman T, et al. Brain fatty acid synthase activates PPARalpha to maintain energy homeostasis. The Journal of Clinical Investigations. 2007;117:2539–2552
  4. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Analytical Biochemistry. 1987;162:156–159
  5. Dowell P, Hu Z, Lane MD. Monitoring energy balance: metabolites of fatty acid synthesis as hypothalamic sensors. Annual Review of Biochemistry. 2005;74:515–534
  6. Gehlert DR. Role of hypothalamic neuropeptide Y in feeding and obesity. Neuropeptides. 1999;33:329–338
  7. Hu Z, Cha SH, Chohnan S, Lane MD. Hypothalamic malonyl-CoA as a mediator of feeding behavior. Proceedings of the National Academy of Sciences of the USA. 2003;100:12624–12629
  8. Kalra SP, Dube MG, Sahu A, Phelps CP, Kalra PS. Neuropeptide Y secretion increases in the paraventricular nucleus in association with increased appetite for food. Proceedings of the National Academy of Sciences of the United States of America. 1991;88:10931–10935
  9. Kochan Z, Bukato G, Swierczynski J. Inhibition of lipogenesis in rat brown adipose tissue by clofibrate. Biochemical Pharmacology. 1993;46:1501–1506
  10. Kochan Z, Karbowska J, Swierczynski J. Unusual increase of lipogenesis in rat white adipose tissue after multiple cycles of starvation-refeeding. Metabolism. 1997;46:10–17
  11. Lane MD, Cha SH. Effect of glucose and fructose on ford intake via malonyl-CoA signaling in the brain. Biochemical and Biophysical Research Communications. 2009;382:1–5
  12. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and 2−ΔΔCT method. Methods. 2001;25:402–408
  13. Loftus TM, Jaworsky DE, Frehywot GL, Townsend CA, Ronnett GV, Lane MD, et al. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science. 2000;288:2379–2381
  14. Lopez M, Tovar S, Vazquez MJ, Nogueiras R, Senaris R, Dieguez C. Sensing the fat: fatty acid metabolism in the hypothalamus and the melanocortin system. Peptides. 2005;26:1753–1758
  15. Lopez M, Lage R, Saha AK, Perez-Tilve D, Vazquez MJ, Varela L, et al. Hypothalamic fatty acid metabolism mediated the orexigenic action of ghrelin. Cell Metabolism. 2008;7:389–399
  16. Marsh DJ, Miura GI, Yagaloff KA, Schwartz MW, Barsh GS, Palmiter RD. Effects of neuropeptide Y deficiency on hypothalamic agouti-related protein expression and responsiveness to melanocortin analogue. Brain Research. 1999;848:66–77
  17. Mercer JG, Moar KM, Logie TJ, Findlay PA, Adam CL, Morgan PJ. Seasonally inappropriate body weight induced by food restriction: effect on hypothalamic gene expression in male Siberian hamsters. Endocrinology. 2001;142:4173–4181
  18. Migrenne S, Magnan C, Cruciani-Guglielmacci C. Fatty acid sensing and nervous control of energy homeostasis. Diabetes and Metabolism. 2007;33:177–182
  19. Mizuno TM, Makimura H, Silverstein J, Roberts JL, Lopingco T, Mobbs CV. Fasting regulates hypothalamic neuropeptide Y, agouti-related peptide, and proopiomelanocortin in diabetic mice independent of changes in leptin or insulin. Endocrinology. 1999;140:4551–4557
  20. Okamoto K, Kakuma T, Fukuchi S, Masaki T, Sakata T, Yoshimatsu H. Sterol regulatory element binding protein (SREBP)-1 expression in brain is affected by age but not by hormones or metabolic changes. Brain Research. 2006;1081:19–27
  21. Ronnett GV, Kleman AM, Kim EK, Landree LE, Tu Y. Fatty acid metabolism, the central nervous system, and feeding. Obesity. 2006;14:201S–207S(Silver Spring)
  22. Salati LM, Clarke SD. Fatty acid inhibition of hormonal induction of acetyl-coenzyme A carboxylase in hepatocyte monolayers. Archives of Biochemistry and Biophysics. 1986;246:82–89
  23. Shimokawa T, Kumar MV, Lane MD. Effect of a fatty acid synthase inhibitor on food intake and expression of hypothalamic neuropeptides. Proceedings of the National Academy of Sciences of the United States of America. 2002;99:66–71
  24. Sucajtys-Szulc E, Goyke E, Korczynska J, Stelmanska E, Rutkowski B, Swierczynski J. Refeeding after prolonged food restriction differentially affects hypothalamic and adipose tissue leptin gene expression. Neuropeptides. 2009;43:321–325
  25. Swart I, Jahng JW, Overton JM, Houpt TA. Hypothalamic NPY, AGRP, and POMC mRNA responses to leptin and refeeding in mice. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. 2002;283:R1020–R1026
  26. Świerczyński J, Goyke E, Wach L, Pankiewicz A, Kochan Z, Adamonis W, et al. Comparative study of the lipogenic potential of human and rat adipose tissue. Metabolism. 2000;49:594–599
  27. Wolfgang MJ, Lane MD. Control of energy homeostasis: role of enzymes and intermediates of fatty acid metabolism in the central nervous system. Annual Review of Nutrition. 2006;26:23–44
  28. Wolfgang MJ, Lane MD. Hypothalamic malonyl-coenzyme A and the control of energy balance. Molecular Endocrinology. 2008;22:2012–2020

PII: S0143-4179(09)00134-6

doi: 10.1016/j.npep.2009.11.005

Neuropeptides
Volume 44, Issue 1 , Pages 17-23 , February 2010

Article Tools

* Image Usage & Resolution